The CSB Cryo-EM Facility offers training and consultation in planning EM experiments, sample preparation and imaging, using equipment and data analysis.<\/p>\n
Before pursuing cryo-EM as an analytical technique, one must have a biochemically pure sample. Sample purity must be improved in each investigator’s laboratory. We recommend discussing the project with our staff to build the final-phases of the purification protocol, since some buffers are not compatible with cryo-EM.<\/p>\n
Visit\u00a0the facility iLab site<\/a> to complete a training or consultation request to discuss your goals with facility staff.\u00a0 Be prepared to discuss research plan by providing information on sample quality, complex size, stability and known structural information.<\/p>\n Information to set up an iLab Account: iLab Account Basics and\u00a0Setting Up a New User Account Guide<\/a>.<\/p>\n If you are new to structural biology research or require more assistance beyond preparing grids and using the microscopes, submit a CSB Consultation form<\/a> for CSB staff scientists to assess how the available CSB facilities can aid your projects.<\/p>\n The CSB Cryo-EM Facility is a shared resource for the Center of Structural Biology (CSB). All users share responsibility for careful, error-free usage of the microscopes and other equipment in the facility. Facility usage can be accessed via\u00a0facility staff<\/a>, unless the user is a facility-certified microscopist. Microscopists must be trained or certified by the facility director. All facility usage must be logged and authorized.<\/p>\n To request access to the Titan Krios, visit the facility\u00a0iLab site<\/a> and complete the Krios Request for Screening and Data Acquisition from under the Request Services tab.<\/p>\n The CSB Cryo-EM Facility provides tools and supplies for common use. Regular facility users are advised to stock and maintain their own tool kits.<\/p>\n Common EM supply vendors:<\/p>\n <\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" Starting a CSB Cryo-EM Project The CSB Cryo-EM Facility offers training and consultation in planning EM experiments, sample preparation and imaging, using equipment and data analysis. Before pursuing cryo-EM as an analytical technique, one must have a biochemically pure sample. Sample purity must be improved in each investigator’s laboratory. We recommend discussing the project with…<\/p>\n","protected":false},"author":1185,"featured_media":0,"parent":128,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"spay_email":""},"tags":[],"acf":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/pages\/1118"}],"collection":[{"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/users\/1185"}],"replies":[{"embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/comments?post=1118"}],"version-history":[{"count":27,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/pages\/1118\/revisions"}],"predecessor-version":[{"id":4492,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/pages\/1118\/revisions\/4492"}],"up":[{"embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/pages\/128"}],"wp:attachment":[{"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/media?parent=1118"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/tags?post=1118"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}CSB Cryo-EM Training<\/a><\/h4>\n
Titan Krios Access<\/h4>\n
EM Tools Kits and Vendors<\/h4>\n
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