{"id":3313,"date":"2021-04-27T14:07:44","date_gmt":"2021-04-27T19:07:44","guid":{"rendered":"https:\/\/www.vanderbilt.edu\/csb\/?p=3313"},"modified":"2021-04-28T10:04:08","modified_gmt":"2021-04-28T15:04:08","slug":"titan-krios-cryo-electron-microscope-fully-operational","status":"publish","type":"post","link":"https:\/\/www.vanderbilt.edu\/csb\/2021\/04\/27\/titan-krios-cryo-electron-microscope-fully-operational\/","title":{"rendered":"Titan Krios Cryo-Electron Microscope Fully Operational"},"content":{"rendered":"
\"\"<\/a>
FSC plot<\/figcaption><\/figure>\n

The CSB Cryo-EM Facility is thrilled to announce that the G4 Titan Krios cryo-TEM, located in the basement of the Engineering and Science Building, is fully operational and accepting biological samples for imaging.<\/p>\n

The Titan Krios utilizes levels of automation to collect high-resolution data for single-particle analysis and cryo-electron tomography. Complete with Gatan BioQuantum K3 and Thermo Scientific Falcon 3 direct electron detectors, this high-throughput configuration can produce more than 4,000 images per day, increasing the speed of and accessibility for studying structural information on the molecular level for a variety of subjects, such as viral infection, DNA replication, ion channels, membrane proteins, vesicle trafficking and bacterial toxins.<\/p>\n

\"\"<\/a>
Slice of the ~2 \u00c5 model showing clear density of aromatic rings<\/figcaption><\/figure>\n

The system has met all performance specifications and in a recent test using an ideal biological sample, produced a 3D density map that revealed molecular detail to a resolution less than 2.0 \u00c5.<\/p>\n

Contact facility directors Melissa Chambers<\/a> or Scott Collier<\/a> for more information. For details on submitting a sample for imaging, please visit the CSB Cryo-EM Facility website<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"

The CSB Cryo-EM Facility is thrilled to announce that the G4 Titan Krios cryo-TEM, located in the basement of the Engineering and Science Building, is fully operational and accepting biological samples for imaging. The Titan Krios utilizes levels of automation to collect high-resolution data for single-particle analysis and cryo-electron tomography. Complete with Gatan BioQuantum K3…<\/p>\n","protected":false},"author":1185,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"spay_email":"","jetpack_publicize_message":"","jetpack_is_tweetstorm":false,"jetpack_publicize_feature_enabled":true},"categories":[1],"tags":[],"acf":[],"jetpack_featured_media_url":"","jetpack_publicize_connections":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/posts\/3313"}],"collection":[{"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/users\/1185"}],"replies":[{"embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/comments?post=3313"}],"version-history":[{"count":12,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/posts\/3313\/revisions"}],"predecessor-version":[{"id":3328,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/posts\/3313\/revisions\/3328"}],"wp:attachment":[{"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/media?parent=3313"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/categories?post=3313"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/tags?post=3313"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}